Cellular Growth Dynamics Affects Allelopathic Activity in Coffee Cell Culture

Cellular growth dynamics and allelopathic activity in coffee cell cultures were examined as follows: First, we compared of seven woody plant calli, Coffea canephora, Derris indica, Ficus carica L., Juniperus conferta, Prunus persica, Punica granatum, Sonneratia ovata, using a modified “sandwich method bioassay” found that callus showed the strongest inhibition to lettuce seedling nearly 90% hypocotyl 96% root. This actively proliferated, with 21-fold increase during five weeks subculture, curve comprising two typical phases: lag phase 0 - 2 culture an exponential 3 5 culture. Allelopathic varied depending on callus. The was detected 1 2-week-old showing 100% inhibitory effect growth. As calli is extremely high, beyond natural level leaves green beans, focused analyzing its aqueous extracts high-performance liquid chromatography. Several prominent peaks, including reference alkaloids, theobromine caffeine, which are known allelochemicals plants, three distinct unknown peaks identified at 270 nm (1 culture). higher value total phenolic content also suggested key biosynthetic pathway relation will be activated phase.